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The Calligraphic Brush Options, as shown in Figure 4.30, are as follows: Name: You can type a name here for your new brush or rename an existing brush (maximum of 30 characters). Angle: You can set the angle of the Calligraphic brush. The angle you should choose depends on what s going to be drawn. To mimic hand-drawn lettering in a calligraphic style, the angle should be set to 45 (or if you re left-handed, it should be set to 45 ). Roundness: This does what you think it does. It sets the roundness of the brush. The higher you make the value, the rounder the brush. Diameter: The diameter value sets the maximum diameter of the brush. Variation: If you choose the Random option from the Angle or Roundness dropdown lists, you can then type a value for Variation. The Variation for the Angle value is in a degree that you want to vary from the original setting for each brushstroke. The Variation for the Roundness is set in percentages. A slider sets the Variation for the Diameter or you can type a number. The Diameter Variation goes from your original value up to the Variation value. This is a great way to simulate a hand-drawn look if you don t have a pressure-sensitive tablet.

Surprisingly, based on the criteria that are described below, we have been able to identify cells with progenitor-like properties from aged (>70 year old) articular cartilage and from cell clusters (chondrones) in osteoarthritic cartilage in addition to those expected from fetal cartilage Interestingly, in terms of potential clinical use in articular repair procedures, there has been an intensive quest for alternative tissue sources (due possibly to the only recent discovery of progenitors from the tissue itself) of cells with chondrogenic potential from a variety of other connective tissues These have included periosteum [O Driscoll and Fitzsimmons, 2001], perichondrium [Arai et al, 2002], bone marrow stromal cells [Vaananen, 2005], adipose tissue [Guilak et al, 2004], and dermal stem cells [Sorrell and Caplan, 2004], to name but a few, and they have been compared in an animal defect model [Hui et al, 2005].

More recently, umbilical cord blood has also been demonstrated to contain cohorts of cells with chondrogenic potential [Moise, 2005] (see 7) In terms of cartilage, it may be the case that virtually any stem cell isolated from connective tissues can be induced to become chondrogenic but the strength and stability of the phenotype vary Even progenitor cells from articular cartilage vary in their chondrogenic capacity Therefore, it appears that chondrocytes generated from stem/progenitor cells either from the same source or differing sources are not equivalent The techniques outlined in this chapter are all based on differential adhesion to bronectin It is common that many stem/progenitor cells have high af nity for bronectin, and our initial isolation of these cells in articular cartilage was partly based on the speci c expression of the extra domain A (EDA) splice variant of bronectin in the surface of cartilage [Dowthwaite et al, 2004].

The aprint function takes as arguments a format string and a variable list of arguments, in the fashion of pri nt. The format string is made up of Unicode characters, and the returned array of bytes is the UTF-8 encoding of the Unicode string. For example, the stringmurmigki,which is made up of eight Unicode characters, is converted to a UTF-8 stream, consisting of 16 bytes, illustrated by the following Limbo program:

To avoid erroneous requests, a simple if statement is used to make sure the tags variable that comes from Flash in fact has a search term. If for some reason the variable is empty, the script exits and no more code is run from that point.

Admittedly, to date, we do not know what the relevance of this particular splice variant is to the progenitor cells Nonetheless, since we are interested in the clinical application of these cells, it is a useful method of isolation for the following reasons If we were to isolate cells based on cell surface-expressed molecules, we would require a uorescence-activated cell sorter (FACS) However, none of these machines (to date) has been accredited for clinical use, and it seems unlikely that this will be the case for some time to come While the use of puri ed ligands offers a better prospect, quality control still remains a problem.